We are looking for a Researcher to participate in our exciting studies of the human protein kinome.

Located at St. Jude Children's Research Hospital in Memphis, Tennesee, this position is part of the Blue Sky Initiative “Seeing the Invisible in Protein Kinases,” an inter-disciplinary and internally funded project in the Department of Structural Biology.

This large-scale project leverages nuclear magnetic resonance (NMR) spectroscopy, complemented with cryoEM, X-ray crystallography, data science and computational approaches, to structurally characterize the conformational landscape of the human kinome. Combined with biochemical and biophysical studies, knowledge of the conformations of kinases is then used to discover new biology regarding kinase function and regulation in cells, understand the structural and functional consequences of disease-associated mutations, and identify novel therapeutic opportunities.

As a member of the team, the candidate will contribute to the project by applying basic molecular biology and protein biochemistry methods, including cloning, PCR, DNA extraction, immunoprecipitation, protein purification, Western blot analysis, macromolecular complex assembly and purification. A key responsibility is preparing high-quality proteins for structural studies of kinases by NMR, and crystallography.

The researcher will be responsible for coordinating efficient laboratory operations, maintaining equipment, keeping accurate data records and notebooks, and performing other duties as assigned or directed in order to meet the goals and objectives of the lab.

Key Responsibilities

• The candidate will use their molecular biology and biochemistry skills to design constructs and purify proteins for structural studies and biophysical characterization.
• Able to troubleshoot protein expression and purification problems.
• Interpret, analyze, and communicate results with supervisors and colleagues.
• Learn and conduct basic biophysical and biochemical experiments.
• Routine ordering of the lab supplies and maintaining the stock
• Prepare media, buffers and common reagents for the protein production and biochemistry work.
• Maintain the laboratory equipment and coordinate the smooth running of the lab.
• Prioritize work and complete assignments in a timely fashion.

Minimum Education and/or Training:

• Bachelor's degree in relevant scientific area is required.

Minimum Experience:

• no additional experience required.

Compensation

In recognition of certain U.S. state and municipal pay transparency laws, St. Jude is including a reasonable estimate of the compensation range for this role. This is an estimate offered in good faith and a specific salary offer takes into account factors that are considered in making compensation decisions including but not limited to skill sets, experience and training, licensure and certifications, and other business and organizational needs. It is not typical for an individual to be hired at or near the top of the salary range and compensation decisions are dependent on the facts and circumstances of each case. A reasonable estimate of the current salary range is $24.00 - $42.00 per hour for the role of Researcher in Kalodimos Lab.

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